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KMID : 0848120140390010015
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International Journal of Oral Biology
2014 Volume.39 No. 1 p.15 ~ p.22
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Nuclear Factor-κB Dependent Induction of TNF-α and IL-1β by the Aggregatibacter actinomycetemcomitans Lipopolysaccharide in RAW 264.7 Cells
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Na Hee-Sam
Jeong So-Yeon
Park Mi-Hee
Kim Se-Yeon
Chung Jin
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Abstract
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Aggregatibacter actinomycetemcomitans is an important pathogen in the development of localized aggressive periodontitis. Lipopolysaccharide (LPS) is a virulent factor of periodontal pathogens that contributes to alveolar bone loss and connective tissue degradation in periodontal disease. Our present study was designed to investigate the cytokine expression and signaling pathways regulated by A. actinomycetemcomitans LPS (Aa LPS). Cytokine gene expression profiling in RAW 264.7 cells was performed by microarray analyses. The cytokine mRNA and protein levels and related signaling pathways induced by Aa LPS were measured by RT-PCR, ELISA and western blotting. Microarray results showed that Aa LPS strongly induced the expression of NF-κB, NF-κB-related genes, inflammatory cytokines, TNF-α and IL-1β in RAW 264.7 cells. NF-κB inhibitor pretreatment significantly reduced the levels of TNF-α and IL-1β mRNA and protein. In addition, the Aa LPS-induced TNF-α and IL-1β expression was inhibited by p38/JNK MAP kinase inhibitor pretreatment. These results show that Aa LPS stimulates TNF-α and IL-1β expression through NF-κB and p38/JNK activation in RAW 264.7 cells, suggesting the essential role of this pathway in the pathogenesis of localized aggressive periodontitis.
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KEYWORD
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NF-κB , Aggregatibacter actinomycetemcomitans , lipopolysaccharide , TNF-α , IL-1β.
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